BIO RAD ZE5 User Manual page 160

Chapter 6 Daily Routine
Table 32. Steps in the QC process
Perform EYE Process
Build Filter Set
Build Channel Manager
Create Workspace
Build Runlist
Set Default PMT Voltages
Add Plots
Start Runlist
Test Validation Event Rate
158 | ZE5 Cell Analyzer and Everest Software
Checks the physical optical filter configuration in the instrument
against the software.
Configures acquisition according to the filter set currently installed
in the instrument.
Configures channels for acquisition.
Creates a workspace containing a FSC x SSC plot and a univariate
histogram for each channel.
Builds a run list (including loader movement, accessing the QC
bead bottle, and bead agitation) for the QC process.
Sets PMT voltages to the values determined at the end of the last
successful QC process.
Adds plots to the workspace.
Initializes and begins acquisition.
Tests whether the system can acquire beads at 150 events per
second.