Decontaminating The System - BIO RAD ZE5 User Manual

Chapter 12 Maintenance
c. Ensure that the liquid solution in each bottle is thoroughly mixed.
To prepare the rinse bottles for decontamination
1. Obtain the following materials:
n Two 4 L sheath bottles
n
One additive bottle
n One cleaner bottle
n
Filtered DI water
2. Add 2.5 L filtered DI water to each 4 L sheath bottle .
3. Add 200 ml filtered DI water to the additve bottle and the cleaner bottle.
To prepare the system for decontamination
u
In the instrument's fluidics chamber, replace both sheath bottles and the additive and cleaner
bottles with those containing the filtered bleach solution.
See Replacing Sheath Bottles on page 146
on page 148 for information about removing and replacing the bottles.

Decontaminating the System

Important: The decontamination process takes approximately 4 hr to complete. During the
process, you will be prompted to
n
Replace both sheath bottles approximately 50 min into the procedure with sheath bottles filled
with 2.5 L of DI water.
n
Replace the additive and cleaner bottles filled with 200 ml of DI water.
n Replace both sheath bottles again approximately 190 min into the procedure with the default
(original) sheath bottles filled to the Fill Line with sheath fluid.
n
Replace the additive and cleaner bottles with the default (original) additive and cleaner bottles
filled to the Fill Line with the appropriate solutions.
Before running the wizard, ensure that the waste bottles are empty. The bottles containing the bleach
solution must be filled no more than halfway.
Important: When handling sheath fluid and DI water bottles, always wear gloves and minimize air
exposure to help avoid contamination.
328 | ZE5 Cell Analyzer and Everest Software
and Replacing Sheath Additive and Cleaner Bottles