Chapter 10 Analyzing, Saving, And Printing Data; File Types In Everest Software; File Structure Of Saved Data - BIO RAD ZE5 User Manual

Chapter 10
You can access the Everest™ Software Analysis tab by first acquiring data in the Acquisition tab and
then sending it to the Analysis tab, or you can directly click the Analysis tab and load a previously
acquired experiment session.
The Analysis tab allows you to create new plots for viewing data, view the PMT voltages that were set
when the data were acquired, view and edit the existing compensation matrix, or run automatic
compensation. From this tab, you can export CSV, FCS, RLST, and ZIP files.
When data analysis is complete, you can transfer the workspace view to the Publish tab, where you
can continue to fine-tune experimental data for presentation.

File Types in Everest Software

There are two main types of files created by Everest Software during sample acquisition: FCS and
RLST files.
FCS files are formatted using the 3.1 standard and thus can be analyzed using compatible third-party
applications such as FlowJo, WinList, and Kaluza. Run list (RLST) files are specific to Everest
Software. They contain all of the information related to an experiment, including fluorophores,
samples, sampling settings, instrument settings, plots, and the compensation matrix.
Run list files are used to:
n Open previously created experiments for re-running or editing
n
Load experiments in the Analysis tab

File Structure of Saved Data

Tip: This section explains where to find saved data, but the best way to obtain FCS files for
analysis in third-party software is to load the experiment and export the data. For more
information, see
The folder for each saved experiment contains a folder for each experiment session. The session
folder contains the run list file and instrument definition file for the session, as well as subfolders
containing detailed experiment data.
Analyzing, Saving, and Printing Data
Exporting FCS and RLST Data Files on page 288.
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