BIO RAD ZE5 User Manual page 352

Appendix A Troubleshooting
Error
High CV in data plots
350 | ZE5 Cell Analyzer and Everest Software
Possible causes
Poor sample preparation
Dirty or clogged flow cell
Air in system
Dirty optical filters
Improper laser delay
Beads have gone bad or expired
Troubleshooting steps
Prepare a new sample.
Follow these steps, testing
after each to determine
whether the problem has
been resolved:
1. Run the unclog process.
See Unclogging the
Sample Line and Probe on
page 322.
2. Run the cleaning process.
See Cleaning the Sample
Line and Probe on page
324.
3. Run the Decontamination
wizard. See
Decontaminating the
System on page 325.
If these steps do not resolve
the issue, contact Bio-Rad
Technical Support.
Stop sample acquisition and
run a stat tube filled with at
least 500 µl DI water.
Inspect and clean filters. See
Cleaning the Optical Filters
on page 334.
Run the QC process again.
See Running Quality Control
on page 156.
Install a new bottle of QC
beads. See Replacing the
QC Beads on page 335.