BIO RAD QX200 Instruction Manual
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BIO RAD QX200 Instruction Manual

Droplet reader and quantasoft software
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QX200 Droplet Reader and
QuantaSoft Software
Instruction Manual
Catalog # : 1864001
1864003

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Summary of Contents for BIO RAD QX200

  • Page 1 QX200 Droplet Reader and QuantaSoft Software Instruction Manual Catalog # : 1864001 1864003...
  • Page 3 Bio-Rad Technical Support Department The Bio-Rad Technical Support Department in the U.S. is open Monday through Friday, 5:00 AM to 5:00 PM, Pacific Standard Time. Phone: 1-800-424-6723, option 2 Email: Support@bio-rad.com (U.S./Canada only) For technical assistance outside the U.S. and Canada, contact your local technical support office or click the Contact us link at bio-rad.com.
  • Page 4 This instrument is for use only by trained personnel. Do not position the equipment so that it is difficult to operate the plug of the power supply. ii | QX200 Droplet Reader and QuantaSoft Software Instruction Manual ii | QX200 Droplet Reader and QuantaSoft Software Instruction Manual...
  • Page 5 Alteration of this instrument voids the warranty and safety certification and creates a potential safety hazard. This instrument is intended for laboratory use only. Bio-Rad Laboratories is not responsible for any injury or damage caused by use of this instrument for purposes other than those for which it is intended, or by modifications of the instrument not performed by Bio-Rad Laboratories or an authorized agent.
  • Page 6 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual...

  • Page 7: Table Of Contents

    1.4 System Setup and General Operation Instructions ....4 Chapter 2. Using the QX200 Droplet Reader ......5 Chapter 3.
  • Page 8 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual vi | QX200 Droplet Reader and QuantaSoft Software Instruction Manual...

  • Page 9: Chapter 1. Qx200 Droplet Digital Pcr System

    Single-cell gene expression analysis ■ ■ For instructions on use of the QX200 Droplet Generator, please refer to the QX200 Droplet Generator Instruction Manual (bulletin 10031907). For information on QX200 AutoDG Droplet Digital PCR System and the Automated Droplet Generator, please refer to the Automated Droplet Generator Instruction Manual (bulletin 10043138).

  • Page 10: System Components

    QX200 Droplet Generator — utilizes proprietary reagents and microfluidics to partition samples into ■ ■ ~20,000 nanoliter-sized droplets QX200 Droplet Reader — following PCR amplification of the nucleic acid target in the droplets, this ■ ■ instrument analyzes each droplet individually using a two-color detection system (set to detect FAM and either VIC, HEX, or EvaGreen ®...
  • Page 11 Pierceable foil plate seals 1814040 Plate sealer PX1 PCR Plate Sealer 1814000 QX200 Droplet Reader Droplet reader oil ddPCR Droplet Reader Oil 1863004 Droplet reader waste bottle Empty waste bottle QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 3...

  • Page 12: Droplet Digital Pcr Workflow

    ■ ■ PX1 Plate Sealer (see Table 1.2). Perform PCR to end point (~40 cycles) using a thermal cycler Read droplets — load the plate into the QX200 Droplet Reader and start your run. The droplet reader sips each ■ ■...

  • Page 13: Chapter 2. Using The Qx200 Droplet Reader

    Using the QX200 Droplet Reader 1. Power on the QX200 Droplet Reader using the switch at the back. Switch on the PC and launch QuantaSoft Software. 2. Check the indicator lights on the front of the droplet reader (Table 2.1). The first two lights at left should be solid green, indicating power is on, there is sufficient oil in the designated oil reservoir, and there is <700 ml in the waste...
  • Page 14 Vendor: Troutt Visual Services Vendor: Troutt Visual Services Fig. 3. Placing the plate holder into the droplet reader. File Name: 17-0767_04 File Name: 17-0767_04 Date: 07/16/17 Date: 07/16/17 6 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual Proof: 4 Proof: 4...
  • Page 15 Project Title: QX200 Images Project Title: QX200 Images Vendor: Troutt Visual Services Vendor: Troutt Visual Services File Name: 17-0767_06 File Name: 17-0767_06 Date: 07/24/17 Date: 07/24/17 Proof: 2 Proof: 2 QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 7...
  • Page 16 8 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual 8 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual...

  • Page 17: Chapter 3. Using Quantasoft Software

    Results (*.qlp) — user-defined plate layout settings and processed data for ■ ■ a ddPCR Plate Comma-separated values (*.csv) — analyzed data in a format that can be ■ ■ assessed using other programs, such as Microsoft Excel QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 9...

  • Page 18: Setup

    2. Enter the file name, then use the well editor (see Section 3.1.1) and experiment editor (see Section 3.1.2) to adjust the settings for your experiment. Click Options to access advanced options for data analysis and setup (see Section 3.1.3). 10 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual...

  • Page 19: Using The Well Editor

    To create or edit an experiment, use the experiment editor (see Section 3.1.2) ■ ■ 3. Select the Supermix from the dropdown menu (required; selection cannot be changed after data collection). QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 11...

  • Page 20: Using The Experiment Editor

    Copy number options appear for CNV experiments only Customize the color codes for experiments (for example, by experiment type, user, etc.) Fig. 10. Experiment editor. Options for a CNV experiment are shown. 12 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual...

  • Page 21: Using The Advanced Options

    Active and analyzed wells are also highlighted in green in the plate map. 4. As each well is analyzed, the data appear across the top navigation area. Once the run is complete, all data are reanalyzed for the final data file. QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 13...

  • Page 22: Analyze

    Results table — summarizes results for wells selected in the well selector ■ ■ Well selector — enables selection of wells for targeted analysis ■ ■ Processed data/graphical display — allows visualization of graphical data from selected wells ■ ■ 14 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual...
  • Page 23 (<10,000). Manually set a threshold and QuantaSoft Software will calculate a concentration, which will appear in the concentration chart. QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 15...

  • Page 24: Viewing Channel Data

    Click Auto Analyze to revert to automatic threshold settings and calculations. Threshold adjustments can also be made in the 2D Amplitude scatter plots (see Section 3.3.2). 16 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual...
  • Page 25 (the threshold is indicated by the pink line and the value in the Set Threshold field; the status of that well in the results table shows as Manual). In Channel 2, the multiple threshold tool is enabled. QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 17...

  • Page 26: Viewing Scatter Plots

    Fig. 19. Viewing scatter plots. Threshold adjustment options available in the scatter plot are shown. Threshold values are indicated by the pink lines in the plot. 18 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual 18 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual...

  • Page 27: Viewing Concentration Data

    (in copies per µl) multiplied by the 20 µl ddPCR reaction input used to make droplets. This value does not appear for merged well data. This column is also exported in the *.csv file. QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 19...

  • Page 28: Viewing Copy Number Data

    Poisson confidence limits. Select y log to convert the y-axis to logarithmic scale (shown). Solid data points (not shown) indicate merged data; open data points (shown) indicate data from single wells. 20 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual...

  • Page 29: Viewing Events

    The simulation mode also allows you to load a demo plate or create a new template and simulate a ddPCR run. ■ Options available in simulation mode Fig. 24. Simulation mode (main screen). QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 21...
  • Page 30 22 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual 22 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual...

  • Page 31: Chapter 4. Specifications And Maintenance

    II (external power supply plugs into standard AC receptacle) Ventilation requirements 5" (12 cm) left and right of machine and 10" (25 cm) behind should be unobstructed for proper ventilation QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 23...

  • Page 32: Maintenance

    Do not replace detachable power cord with an uncertified or an inadequately rated cord. Since the QX200 Reader contains moving parts, fluids, tubing, and pumps, we strongly recommend annual preventive maintenance on the instrument. 4.2.2 Replacing Droplet Reader Oil and Removing Waste Replace the droplet reader oil and waste receptacle as needed.
  • Page 33 Fig. 26. Replacing droplet reader oil and removing waste. Slide out the carriage containing the droplet reader oil and waste receptacles to remove and replace the bottles (left). After replacing oil, click Prime to fill the lines; other instrument maintenance routines are for use by field specialists only. QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 25...

  • Page 34: Error Codes

    Windows on the host computer. If the error persists, contact Technical Support. Tips: The computer sees the QX200 Droplet Reader as an attached “removable disk” drive. Sometimes Windows updates will stall when Windows tries to query the reader; this problem can easily be solved by making sure you have the instrument powered off or disconnected when running Windows updates on the computer.

  • Page 35: Appendix A Ordering Information

    QX200 Droplet Digital PCR System, includes droplet generator, droplet reader, laptop computer, software, associated component consumables 1864002 QX200 Droplet Generator, includes droplet generator, 1 box of 24 cartridges, 1 pkg of 24 gaskets, 2 cartridge holders, 1 power cord 1864003...
  • Page 36 2x ddPCR Supermix for Residual DNA Quantification 17000032 ddPCR E. coli Residual DNA Quantification Kit, 200 x 20 µl reactions, includes 20x E. coli RDQ assay and 2x ddPCR Supermix for Residual DNA Quantification 28 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual...
  • Page 37 Consumables and Accessories for QX200 ddPCR System Only 1864005 Droplet Generation Oil for EvaGreen ® , 14 ml (2 x 7 ml) 1864006 ® , 70 ml (10 x 7 ml) Droplet Generation Oil for EvaGreen QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 29...

  • Page 38: Qx200 Droplet Reader System Maintenance Log

    30 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual...
  • Page 40 Bio-Rad Laboratories, Inc. Web site bio-rad.com USA 1 800 424 6723 Australia 61 2 9914 2800 Austria 43 01 877 89019 Life Science Belgium 32 03 710 53 00 Brazil 55 11 3065 7550 Canada 1 905 364 3435 China 86 21 6169 8500...

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