Setting Up Compensation Controls - BIO RAD ZE5 User Manual

Cell analyzer and everest software
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Chapter 7 Creating Experiments and Workspaces

Setting Up Compensation Controls

To use automatic compensation in Everest Software, activate the compensation template. This tool
creates control positions that integrate with the automatic compensation process. These control
positions are automatically added to the plate map.
To set up compensation controls
1.
In the Template area of the Selected Fluorophores panel, select the pulse parameter (Area or
Height) that you want to compensate.
Note: You must select this parameter first; the Area and Height buttons are disabled after the
compensation template is activated.
2.
To include a negative control tube or well in the experiment, click the Negative Control toggle so
that it is ON.
182 | ZE5 Cell Analyzer and Everest Software

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