BIO RAD ZE5 User Manual page 395

Setup mode
sheath fluid
shortpass filter
stat tube
threshold
time (parameter)
track region percentage
trigger
width
workspace
Typically used during PMT voltage adjustment and setting up regions and
gates, Setup mode can be used to run any sample continuously without
requiring adherence to the limits set in the run list.
A carrier fluid used to hydrodynamically focus the sample stream for proper
interrogation by the laser beam(s). For the ZE5 Cell Analyzer, this is typically
DI water containing a sheath additive, but a saline solution can also be used.
An optical component, usually placed in front of a detector, that allows
passage of a range of wavelengths of light below a specified wavelength while
absorbing or reflecting the rest.
A single tube (as opposed to a rack of tubes) that can be used for quick
sample acquisition without requiring experiment configuration in the Everest
Experiment Builder.
The threshold is set using the trigger parameter(s) and is the level above
which signal must fall to be classified as an event. The value is reported as a
percentage of the total range of signal intensities in that detector. It is not
efficient to analyze every single particle that passes through the interrogation
point — samples contain debris that would inundate the dataset and drown out
the population(s) of interest. Additionally, the threshold is helpful in eliminating
irrelevant optical noise from the data.
Each event is time-stamped when analyzed. All events can be viewed with
respect to when they occurred during the sample acquisition.
A percentage that can be assigned to a region in a plot. During acquisition, if
the percentage of events drops below the specified number, acquisition pauses
and the user is notified. This feature is useful in monitoring for clogs or sample
disturbances during acquisition.
The trigger parameter is the initial parameter of detection that signals the
system that a particle of interest is present. The trigger, combined with the
threshold setting, defines real events that should be detected and analyzed.
Typically, a scatter parameter is selected for the trigger because it identifies
all particles above a given size regardless of the fluorescent signal. However,
any parameter or combination of parameters can be used as a trigger.
See pulse width on page 392.
A set of plots and regions that facilitate data analysis and interpretation.
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