BIO RAD ZE5 User Manual page 70

Chapter 4 Everest Software
When you add a fluorophore to the Selected Fluorophores list, Everest Software activates a
recommended detector to be used as an acquisition parameter. The software populates the parameter
Name box with the fluorophore name. You can edit the name of the parameter. Any changes to the
parameter names will carry through to the plot axes and filter names. You can make additional
parameter selections if needed.
Tip: The only parameters that are activated are those for which fluorophores have been selected.
Therefore, only data from the enabled (active) parameters will be collected during acquisition.
Height (24-bit resolution), area (24-bit resolution) and width (17-bit resolution using linear
interpolation at half height) are collected for all active parameters. All signals are collected as raw
digital values from the analog-to-digital converters (ADCs) in linear mode and are uncompensated.
Logarithmic scaling is performed by Everest Software after the data have been received by the
software.
68 | ZE5 Cell Analyzer and Everest Software