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experimental sample plots, both axes should be designated as Comp if you want to view
compensated data.
To perform manual compensation by manipulating the plots directly
1.
Point to one of the compensation plots.
2.
When the pointer changes to a hand, drag it over the positive population to increase
compensation and move the population out of the spillover channel. (Drag the population towards
the x-axis so that the median matches that of the negative population on the y-axis.)
Tip: If the hand is in a region, it will drag the region instead of the population. Ensure that the
hand is outside of a region before dragging it.
3.
Repeat
Step 1
and
Step 2
Editing the Compensation Matrix
You can adjust compensation by editing the compensation matrix either in the Acquisition tab or in the
Analysis tab.
Note: If you plan to perform manual compensation, you should understand how to match medians
to ensure that you apply accurate compensation values.
To adjust compensation by editing the compensation matrix
1.
Open the compensation matrix by clicking View Compensation Matrix in the Comp section on the
toolbar.
2.
Select the appropriate pulse parameter (area or height).
3.
Directly adjust the spillover values by editing values in the matrix.
for each single-stained control.
Adjusting Compensation Manually
User Guide | 299
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