Chapter 9 Applying Fluorescence Compensation; Setting Up The Experiment - BIO RAD ZE5 User Manual

Chapter 9 Applying Fluorescence Compensation
Everest™ Software offers simplified workflows for both manual and automatic compensation. To
perform automatic compensation, acquire single-color controls using the Everest compensation
template. You can then adjust regions to define the positive populations in all controls' channels and
initiate the automatic compensation calculation. Everest Software also offers automatic region
determination, so that you do not need to manually adjust the gates to define the positive populations.
Tip: Everest Software permits visualization of both compensated and uncompensated data in the
same workspace. Be sure to select the Comp parameter for plot axes so that the compensation
matrix is applied to the data.
For information on applying compensation to multicolor experiments, see
Color Immunophenotyping

Setting Up the Experiment

Before applying compensation in Everest Software (either automatically or manually), set up the
workspace to streamline the compensation process. For information about the Compensation
template, see Setting Up Compensation Controls on page
1. Set up an experiment by selecting fluorophores and activating detectors as described in
7, Creating Experiments and
Experiment.
Workspaces.
Appendix B, Example 9-
182.
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