Glossary - BIO RAD ZE5 User Manual

Glossary

acquisition
Acquisition mode
area
bandpass filter
compensation
cycle mode
dichroic filter
event
event rate
experiment
EYE
Process in which sample data are gathered from the PMTs, processed, and
sent to and displayed in Everest™ Software.
In this mode, samples are processed following the programmed steps in the
run list. This mode is typically used after running samples in Setup mode.
The area under the curve of the pulse, as analyzed by the electronics.
An optical component, usually placed in front of a detector, that allows
passage of wavelengths of light within a specified range while absorbing the
rest.
A mathematical method used to correct the emission overlap from one
fluorophore into the emission channel of another fluorophore. Compensation
can be applied manually or automatically.
Shows a current "snapshot" of data defined within the time (in seconds)
specified by the user. Data are automatically refreshed. Cycle mode is
primarily used while adjusting PMT voltages, regions, and gates in Setup
mode.
An optical component, usually part of the detection path, that allows passage of
a range of wavelengths of light while reflecting the rest. Dichroic filters can
either be longpass or shortpass.
Any signal pulse with an intensity above the threshold in the trigger parameter
(s). See trigger on page 393
The number of independent electronic events above the threshold in the trigger
parameter. The event rate is dictated by the number of particles that pass
through the interrogation point per second; however, it is not always equal to
cells per second — debris, cell clumps, and other material also contribute to
the event rate as they pass through the laser.
Another name for a run list.
See ZE5-EYE on page 394.
and threshold on page 393.
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