Fluidics Issues; Software Issues - BIO RAD ZE5 User Manual

Cell analyzer and everest software
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Fluidics Issues

Error
System suddenly
shuts down

Software Issues

Error
Unable to click the
Play button
Possible causes
Bulk fluidics bottles empty
DI water uptake line and filter
in wrong position
Possible causes
Sample back flushing or
previous run not complete
Run list error
Loader door open or sample
plate inserted incorrectly
Troubleshooting steps
Empty the waste and refill DI water bottles as
necessary. See
Refilling Bulk Fluidics on
page
143.
Ensure that the filter on the DI water uptake
line falls into the lower corner of the bottle on
the side away from the cap. Refer to the
figures in
Replacing Sheath Bottles on page
146.
Troubleshooting steps
Wait for the process to complete.
Validate that everything has been properly
selected when setting up a run list.
1. Stop the acquisition process.
2. Open the loader door (if it is not already
open).
3. Ensure that the sample tube rack or plate is
seated correctly.
4. Close the loader door.
5. Proceed with running the experiment.
Fluidics Issues
User Guide | 343

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