automatically. If this option is selected, Everest Software respects the regions that you
configured in the parameters' histograms to determine positive and negative populations for
compensation. It adjusts the regions in the other plots for each control to match your regions.
n
Only Current Sample — If this option is selected, Everest Software performs compensation
only for the currently selected sample. Spillover of this channel into all other channels will be
corrected only for this sample.
n
Include Invalid Samples — Everest Software evaluates the quality of compensation controls
during the automatic compensation process. If this option is selected, Everest Software
includes controls found to be invalid according to the algorithms that segment the positive
population from the negative population. If this option is not selected, Everest Software
excludes them. Excluding invalid controls might be necessary if there was no clear
separation between positive and negative populations, or if a sufficient number of events
could not be obtained for a particular single color control.
7.
Click Calculate to initiate automatic compensation.
8.
After the compensation process finishes, open the compensation matrix to check the values.
Each row in the matrix corresponds to a channel that contributes spillover signal to other
detectors. Each column in the matrix corresponds to a channel that receives spillover signal from
other detectors. Cells in the matrix are shaded green, yellow, and red to indicate increasing
percentage values.
9.
Review the compensated control plots.
Adjusting Compensation Automatically
User Guide | 297
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