BIO RAD ZE5 User Manual page 359

Error
Weak or no fluorescence
Possible causes
Not enough antibody used during
sample preparation
Intracellular targets insufficiently
labeled
Incorrect fluorophore selection
Poor compensation
Reagent old or degraded
Antibodies are not compatible
Lasers turned off
Lasers misaligned
Acquisition/Event Issues
Troubleshooting steps
Verify antibody titrations and
prepare a new sample.
Ensure correct techniques
are used to fix cells.
Evaluate sample preparation
and experiment setup.
Run the compensation
process. See Adjusting
Compensation Automatically
on page 295.
Antibody might not have been
stored in the proper
conditions (refrigerated and
kept in the dark).
Verify that the secondary
antibody used has been
grown against the species in
which the primary antibody
has been grown.
Turn on lasers in software.
See Configuring Instrument
Settings on page 257.
Contact Bio-Rad Technical
Support.
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