Adding Diagonal Separators To Plots - BIO RAD ZE5 User Manual

To remove hit detection from a region
1. Point to a plot or histogram that contains the region that uses hit detection.
The plot and histogram toolbar appears.
2. Click Assign Hit Regions.
3. In the Available Hit Region dialog box, clear the checkbox of the region for which you want to
remove hit detection.
4. Point outside the plot or histogram to save your changes.
The target symbol is removed from the region and the region is no longer used for hit detection.

Adding Diagonal Separators to Plots

A diagonal separating line in a density plot can assist you in determining fluorescence overlap and in
setting PMT voltages for a compensation control. In a typical scenario, the parameter to be
compensated is shown on the x-axis (as in the plots that Everest Software creates automatically for
compensation controls). If a positive population falls below the diagonal line, this indicates that the
control sample is brighter in the intended channel than in the channel shown on the y-axis. Thus, the
required compensation will be less than 100%. If a positive population falls above the diagonal line,
this indicates that the control sample is brighter in a channel other than the intended one and will
need more than 100% compensation.
To add a diagonal separator
1. Point to the plot to which you want to add diagonal aseparator.
The plot toolbar appears.
2. Click Add Diagonal Separation.
A diagonal line appears on the plot.
Using Plot and Histogram Tools
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