Pausing, Stopping, And Resuming Samples And Experiments; Pausing Sample Acquisition; Stopping Sample Acquisition; Using Pause After In An Experiment - BIO RAD ZE5 User Manual

Chapter 8 Acquiring Samples

Pausing, Stopping, and Resuming Samples and Experiments

There are different ways to pause or stop sample acquisition and experiments, depending on when
you plan to resume.
Note: Pausing or stopping during high-throughput sample runs is not recommended; any
samples in the line that have not yet been analyzed will be backflushed to waste. If a pause or
stop is necessary, the ideal time is between samples in standard sampling mode.

Pausing Sample Acquisition

Note: This option is available only when acquiring in Setup mode and for stat tubes.
Pausing sample acquisition pauses the sample pump. The probe stays in the sample. If recording is
taking place, the FCS data file is not closed. You can use this method if you are making adjustments
but have limited sample; you can pause while trying to figure out gating, for example, then resume to
complete the data file.
To pause sample acquisition
u
In the Instrument Control pane in Setup mode, click Pause Run List.
To resume sample acquisition after a pause
u
In the Instrument Control panel in Setup mode, click Resume Run List.

Stopping Sample Acquisition

Stopping sample acquisition stops the sample pump. The probe exits the sample and is washed, if
wash has not been disabled for the sample position. If recording is taking place, the FCS data file is
closed.
To stop sample acquisition
u
In the Instrument Control panel, click Stop.
To restart sample acquisition after a stop
u
In the Instrument Control panel, click Play.

Using Pause After in an Experiment

In Acquisition mode, to pause sample acquisition at a certain point in the experiment, you can insert a
pause step into the run list. Sample acquisition stops until you are ready to restart it manually. You can
use this method to pause after running compensation controls, or to verify results obtained so far.
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