BIO RAD ZE5 User Manual page 196

Chapter 7 Creating Experiments and Workspaces
n Normal (default) — outside wash 0.25 sec; inside wash 1.0 sec
n
Full — outside wash 0.25 sec; inside wash 2.0 sec
Note: The wash option values are not editable.
Important: Wash is turned on by default. Bio-Rad strongly recommends that you leave it on for all
positions, especially when using high-throughput sampling mode.
Agitation Settings
You can activate agitation for any position. The default and minimum time is 5 sec. The speed and
radius of agitation automatically change with the sample media type.
Tip: It might not be necessary to activate agitation for each well. For the highest throughput rate, it
might be necessary to agitate only a few times within the plate.
Positions in the plate map in which agitation is activated appear as
High Throughput, Pause After, Volumetric Counting, and Return Sample
Settings
By default, all settings are turned off. You can apply any setting to individual sample wells on the plate.
Note: Pause After, Volumetric Counting, and Return Sample settings are available only when
high-throughput mode is off.
194 | ZE5 Cell Analyzer and Everest Software