BIO RAD ZE5 User Manual page 41

Optics
For more information about accessing filters, see Optical Filter Access Door on page 46.
Optical Mirror and Filter Types
Optical mirrors and filters are coated pieces of glass that are designed and configured in specific
patterns to efficiently steer light to the correct detector. Mirrors direct all the light down the detection
path and are not user changeable.
Longpass and shortpass filters can be dichroic or normal incidence. Dichroic filters reflect light that is
not permitted to pass through them. They are typically placed at a 45-degree angle to the incident light
and are used to direct light around the detection path.
Normal incidence longpass filters and bandpass filters typically absorb light that is not permitted to
pass through them. They are placed directly in front of detectors to determine the specific range of
wavelengths that the detector measures.
By analyzing the different detected bands of light, it is possible to examine multiple properties of each
particle.
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