BIO RAD ZE5 User Manual page 354

Appendix A Troubleshooting
Error
352 | ZE5 Cell Analyzer and Everest Software
Possible causes
Laser or lasers off
Laser or lasers not functioning
properly
Events below threshold or
threshold set too high
Threshold not set correctly
PMTs set too high or too low to
see data
Troubleshooting steps
Turn on the relevant lasers in
software. See Configuring
Instrument Settings on page
257. Modify the experiment in
the Experiment Builder so
that required fluorophores are
activated to ensure that
lasers are on when the run
list is initiated. See Selecting
Fluorophores on page 168.
Contact Bio-Rad Technical
Support for help.
Adjust the trigger PMT
voltage or decrease the
threshold percentage. See
Configuring Instrument
Settings on page 257.
Change threshold value. See
Configuring Instrument
Settings on page 257.
Edit PMT voltages in Setup
mode or change trigger
threshold in order to
visualize data. See
Configuring Instrument
Settings on page 257.