Configuring Hit Detection - BIO RAD ZE5 User Manual

To compare samples using heat maps
1. During acquisition or analysis, point to a position on the plate map.
2. View the heat map that appears for the position.
The percentage for the region is shown on each pie slice.
To remove regions from a heat map
1. Point to the plot or histogram that contains the region(s) assigned the heat map.
The plot and histogram toolbar appears.
2. Click Assign Heat Map Region.
3. In the Available Heat Map Regions dialog box, clear the checkbox for each region that you want
to remove from the heat map.
4. Point outside the plot or histogram to save your changes.
The pie chart symbol is removed from the region and the region is no longer applied to the heat
map.

Configuring Hit Detection

In hit detection mode, Everest Software provides real-time feedback regarding whether a population is
present or absent in a given region for each tube or well. You can use hit detection to classify sample
positions as hits only if events in a particular region exceed a threshold that you specify. The region is
assigned as a "hit region." This is helpful if you are trying to determine which wells or tubes contain
positive populations or cells that are of interest.
Using Plot and Histogram Tools
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