Pausing After A Tube Or Well; Setting Volumetric Counting On Samples - BIO RAD ZE5 User Manual

Cell analyzer and everest software
Table of Contents

Advertisement

Chapter 7 Creating Experiments and Workspaces
Note: In high-throughput Acquisition mode, the Pause After, Volumetric Counting, and Return
Sample functions are disabled. High-throughput mode does not support event limits or gate limits,
only volume limits can be used. All samples in a high-throughput run must use the same target
flow rate.

Pausing after a Tube or Well

Note: Pause After is disabled when high-throughput mode is activated.
Pause After can be useful when applying automatic compensation. Activate Pause After on the last
compensation control position in the experiment. Acquisition automatically stops after the file for that
control is recorded. You can then navigate to the Analysis module to perform automatic compensation
and then return to the experiment to acquire and record sample files along with the compensation
matrix, which are exported in the FCS file for analysis in third-party software.
To pause after a tube or well
1.
In the plate map, select the relevant positions.
2.
Click the Pause After button.
The run list pauses after the applicable position has been acquired. Positions for which pause
after is activated are marked with an image in the plate map. For more information, see
Images on page
76.

Setting Volumetric Counting on Samples

To verify the number of cells in a specified volume of each sample, activate Volumetric Counting.
Activating Volumetric Counting automatically activates Return Sample. Excess sample in the line
returns to the tube or well after target volume is acquired.
Note: You can set Return Sample to OFF if necessary. However, when using Volumetric Counting
with Return Sample set to OFF, you might experience sample loss. If sample loss is a concern,
run Volumetric Counting with Return Sample set to ON.
200 | ZE5 Cell Analyzer and Everest Software
Position

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the ZE5 and is the answer not in the manual?

Questions and answers

Table of Contents

Save PDF