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Related Manuals for BIO RAD QX100
Summary of Contents for BIO RAD QX100
- Page 1 QX100 Droplet Generator ™ Instruction Manual Catalog #186-3002...
- Page 2 Eppendorf AG. by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 6,767,512 and 7,074,367. Copyright © 2012 by Bio-Rad Laboratories, Inc. All rights reserved. QX100 Droplet Generator Instruction Manual...
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Page 3: Table Of Contents
Droplet Generator 1.1 Introduction 1.2 QX100 Droplet Generator 1.3 Installation and General Operation Droplet Generation 2.1 Sample Preparation 2.2 Operation of the QX100 Droplet Generator 2.3 Preparation for PCR Specifications and Maintenance 3.1 Specifications 3.2 Maintenance Appendix A Ordering Information... - Page 4 Use only the power cord supplied with the instrument, using only the plug adaptor that corresponds to the electrical outlets in your region. Use of unapproved supermixes may harm the instrument and voids the warranty. QX100 Droplet Generator Instruction Manual...
- Page 5 Nitrile gloves are made of copolymer and provide protection from chlorinated solvents such as trichloroethylene ■ ■ and tetrachloroethene; they offer protection when working with oils, greases, acids, and caustic substances QX100 Droplet Generator Instruction Manual...
- Page 6 QX100 Droplet Generator Instruction Manual...
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Page 7: Qx100 ™ Droplet Generator
■ ■ Next-generation sequencing sample quantification ■ ■ This manual covers use of the QX100 droplet generator and preparation for PCR. For information on the QX100 droplet reader, please refer to bulletin 10026321. QX100 Droplet Generator Instruction Manual... -
Page 8: Qx100 Droplet Generator
96-well PCR plate and amplified to end point using a standard thermal cycler. When cycling is complete, the plate is loaded into the QX100 droplet reader. The droplet reader sips each sample, singulates the droplets, and streams them in single file past a two-color detector. The detector reads each droplet and determines which contain a target (+) and which do not (–). -
Page 9: Installation And General Operation
Eppendorf 951023078 8-cap strips 1.3 Installation and General Operation Connect the QX100 droplet generator to a power source using only the power cord provided. Ensure the ■ ■ ground is reliably connected before plugging in the instrument Leave 10" (5 cm) space clear behind and 5" (2.5 cm) clear to the right and left for proper ventilation ■... - Page 10 Chapter 1 QX100 Droplet Generator QX100 Droplet Generator Instruction Manual...
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Page 11: Droplet Generation
Use standard lab precautions to avoid contamination of the reaction mix and ■ ■ sample: wear gloves, clean pipets, work in a clean area such as a PCR hood, and use low protein binding tubes QX100 Droplet Generator Instruction Manual... -
Page 12: Operation Of The Qx100 Droplet Generator
Droplet Generator ™ The QX100 droplet generator prepares droplets for eight samples at a time. Droplet generation takes 2 minutes for each set of eight samples (20–30 minutes for a 96-well plate). All 8 sample wells in the DG8 droplet generator cartridge must contain sample (or 1x buffer ■... - Page 13 4. Dispense the droplet generator (DG) oil in the reagent trough. Use 700 μl DG oil per 8 samples (one DG oil bottle contains enough oil for a 96-well PCR plate). Reagent trough and droplet generator oil. QX100 Droplet Generator Instruction Manual...
- Page 14 Correct placement of the gasket over the cartridge holder. 7. Place the cartridge holder into the QX100 droplet generator and initiate droplet generation: a. Open the instrument by pressing the button on the green top b.
- Page 15 Keep the DG8 cartridge in the holder. Table 2.1. Status indicator lights on the QX100 droplet generator. If the central LED flashes amber, the gasket is not placed on the holder correctly or is missing and no seal was made. If the right LED flashes amber, a process error occurred because the volume is too low in at least one well.
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Page 16: Preparation For Pcr
Cover the plate (for example, with 8-cap strips or the lid from a pipet tip box) as you work to prevent evaporation and contamination with particulates. Aspirating droplets from the DG8 cartridge. Dispensing droplets into a 96-well PCR plate. QX100 Droplet Generator Instruction Manual... - Page 17 Chapter 2 Droplet Generation 2. Seal the PCR plate wells with foil immediately after transferring droplets to avoid evaporation. Use Easy Pierce foil plate seals (compatible with the needles in the QX100 droplet reader) and a compatible thermal plate sealer.
- Page 18 Chapter 2 Droplet Generation QX100 Droplet Generator Instruction Manual...
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Page 19: Specifications And Maintenance
2 (indoor use) Installation category II (external power supply plugs into standard AC receptacle) Ventilation requirement 5" (12 cm) left and right of machine and 10" (25 cm) behind should be unobstructed for proper ventilation QX100 Droplet Generator Instruction Manual... -
Page 20: Maintenance
Droplets made with Bio-Rad master mix have antimicrobial properties, but microbial growth is possible. The waste profile should contain the following: fluorinated hydrocarbons, water, fluorescent dye (from probes), protein, and nucleic acids. The droplet generator is not intended to be used with biohazardous material. QX100 Droplet Generator Instruction Manual... -
Page 21: Appendix A Ordering Information
DG8 Cartridges for QX100 Droplet Generator, 1 pkg of 24 cartridges 186-3052 Buffer Control Kit, 2 x 4.5 ml bottles 2x buffer, for use with QX100 Droplet 186-3009 DG8 Gaskets for QX100 Droplet Digital PCR system Generator, 1 pkg of 24 gaskets... - Page 22 Thermal Cycler with ™ 96-Well Fast Reaction Module, includes C1000 Touch thermal cycler chassis, 96-well fast reaction module, USB flash drive 186-1096 T100 Thermal Cycler, includes 96-well ™ thermal cycler, power cord, tube support ring QX100 Droplet Generator Instruction Manual...
- Page 23 QX100 Droplet Generator Instruction Manual...
- Page 24 Bio-Rad Laboratories, Inc. Life Science Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 11 5044 5699 Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00 Group France 01 47 95 69 65 Germany 089 31 884 0 Greece 30 210 9532 220 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 91 124 4029300 Israel 03 963 6050 Italy 39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 0318 540666...