Fluidics System - BIO RAD ZE5 User Manual

Fluidics System

The ZE5 fluidics system consists of the bulk fluidics, sample loader, and flow cell. The system supplies
sheath fluid and sample to the flow cell and collects the waste for proper disposal. Sheath fluid
typically consists of DI (deionized) water to which sheath additive is added; however, a saline solution
can be used instead. Laminar sheath flow carries the sample core through the center of the flow cell,
where the particles to be measured are intercepted by laser beams. For detailed information about the
hydrodynamic focusing that occurs in the flow cell, see
Four large (4 L) and two small (450 ml) bottles are located in the instrument's bulk fluidics chamber.
The two large bottles with blue caps contain DI water; the two large bottles with red caps collect waste.
Mounted below the four large bottles, the small bottle with the blue cap contains system cleaner, and
the small bottle with the white cap contains sheath additive.
Caution: Biohazard! Maintain biosafety at all times while operating this instrument.
Consult with your local safety officer or review local, state, and federal regulations to
ensure proper handling and disposal of biohazardous substances.
Flow Cell on page 33.
Fluidics System
User Guide | 27