BIO RAD Ultramark User Manual
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BIO RAD Ultramark User Manual

BIO RAD Ultramark User Manual

Microplate reader
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Ultramark
Microplate Reader
™
User Manual
410-0164 Rev B

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Summary of Contents for BIO RAD Ultramark

  • Page 1 Ultramark Microplate Reader ™ User Manual 410-0164 Rev B...
  • Page 2 Bio-Rad. Ultramark is a trademark of Bio-Rad Laboratories. The Bio-Rad logo is a registered trademark of Bio-Rad Laboratories. All other trademarks and registered trademarks are of their respective...
  • Page 3 This warranty does not extend to any instruments or parts thereof that have been subject to misuse, neglect, or accident, or that have been modified by anyone other than Bio-Rad or that have been used in violation of Bio-Rad instruction.

  • Page 5: Table Of Contents

    Table of Contents 1. General Information....................1 Introduction........................1 Ultramark Specifications....................1 2. Equipment Set-up and Installation................3 Unpacking the Ultramark Microplate Reader ..............3 SCSI II Cable Connection ....................3 Power Connection ......................3 Location of the Instrument....................4 3. Using the Ultramark....................5 Loading a Plate........................

  • Page 7: General Information

    You can use it to perform standard colormetric ELISA and kinetic readings for general cell screening with 6, 12, 24, 96, 384, and 1536-well microplate formats. The Ultramark has a 10-magazine filter holder with a working wavelength range of 260–750 nm and an incubation temperature range up to 42 degrees Celsius.

  • Page 9: Equipment Set-Up And Installation

    The SCSI II port is located on the back of the instrument. There are two connectors, which allows the user to connect more than one SCSI instrument in a chain. If the Ultramark is the last instrument in the chain, make sure that the termination switch (next to the SCSI connectors) is in the “ON” position.

  • Page 10: Location Of The Instrument

    Location of the Instrument The Ultramark microplate reader is a precise optical measuring instrument. The following conditions must be met to ensure maximum sensitivity: • Place the instrument on a flat surface that is free of vibration. • Do not place the instrument in direct sunlight.

  • Page 11: Using The Ultramark

    Plate readings are controlled entirely by Microplate Manager. See Chapter 5 of the Microplate Manager User Guide for instructions. To stop a plate reading that is in progress, press the Stop button on the front panel of the Ultramark or click on the Stop button in the Microplate Manager Protocol dialog box.

  • Page 13: Changing Filters In The Ultramark

    The filter arm will retract. Now turn off the Ultramark. When installing new filters in the Magazine, make sure that each filter is oriented in the Magazine with the numbered side facing the rear of the Ultramark and the shiny side facing the front of the Ultramark.
  • Page 14 The Filter Magazine must be securely fastened to the base to avoid serious problems. Replace the side cover and fasten it with the thumbscrews. Restart the Ultramark. Finally, be sure to enter the new filter information into Microplate Manager (see section 2.7 of the...

  • Page 15: Recovering From A Filter Error

    5. Recovering from a Filter Error There are three filter error messages that can appear in the front panel of the Ultramark: • Filter Memory Error • Filter Set Error • Gain Mes-2 Error To correct these errors: Press any button on the front panel of the Ultramark to enter the Filter Set mode.

  • Page 17: Recovering A Dropped Filter

    6. Recovering a Dropped Filter To recover a filter that has dropped inside the instrument, perform the following steps: 1. Turn power off. 2. Loosen thumbscrews on side panel and remove panel.
  • Page 18 3. Remove thumbscrew holding filter magazine in place. 4. Remove filter magazine. 5. Reach inside instrument, locate dropped filter, and remove. 6. Reinsert filter into clip in magazine, replace the magazine and side panel, and turn the instrument back on. 7.

  • Page 19: Disinfection Procedure

    The instrument must be disinfected before you send it back to Bio-Rad Laboratories for service. For safety reasons, you must fill out a Disinfection Certificate and include it with the instrument.

  • Page 21: Error Codes

    8. Error Codes The following error codes appear in the message window of the Ultramark. Error Message Probable Cause Recommended Solution Memory(SRAM) An empty battery. Contact Bio-Rad. ROM replaced. Not a problem. SRAM chip trouble. Contact Bio-Rad. Memory(DRAM) DRAM chip trouble.
  • Page 22 Probable Cause Recommended Solution Xe flash The Xenon Lamp did not flash. If this occurs only a few times a year, it is no problem. Hi-Voltage power supply Contact Bio-Rad. damaged. Xenon Flash Lamp damaged. Contact Bio-Rad. Gain Mes-1 Measuring electronics Contact Bio-Rad.
  • Page 23 Contact Bio-Rad. DPR R/W SCSI SCSI chip damaged. Contact Bio-Rad. SCSI bus Data communication problem The Ultramark is not the source of the between the Ultramark and problem. Check your computer. your computer. Bus Rst SCSI The termination switch may be Turn the SCSI termination switch on off.

  • Page 25: Product Information

    9. Product Information 170-9500 Ultramark Microplate Imaging System, 110/230 V, Includes 10 Filters (260, 280, 340, 405, 415, 490, 570, 630, 655, and 750 nm) 170-9501 Feeder/Stacker 170-9518 1536 Well Microplate, 25 170-9519 96 Well UV Transparent Microplate, 25 170-9520...

  • Page 27: Order Form-Emission Filter

    Filter marking Reflective side 7.0 +0/-0.25mm dia. 4.9 + 0.1mm Absorbing side 14.0 +0/-0.25mm dia. FAX order form to: Omega Optical 3 Grove St. P.O. Box 573 Brattleboro, VT 05301 Phone: 802/254-2690 FAX 802/254-3937 Bio-Rad order form 410-0170 Rev. A...

  • Page 29: Disinfection Certificate

    Disinfection Certificate This instrument and its inventory have: (1) never been in contact with any dangerous biological material, or (2) been disinfected according to the instructions contained in the operating manual for this instrument. Name (please print): Firm name (please print): Signature: Date:...

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