Preparation Of The Flow Cell - Agilent Technologies 1100 Series Reference Manual

Preparation of the Flow Cell

1 Flush the flow cell with water.
2 Remove the inlet capillary from the flow cell.
3 Take the syringe and fix the needle to the syringe adapter.
4 Suck about 1.0 ml of the calibration sample into the syringe.
5 Keep the syringe in a horizontal position.
6 Remove t#he needle.
7 Add the filter to the syringe and fit the needle to filter.
sample filter
Figure 40
8 Lift the needle tip and carefully eject approximately 0.5 ml to remove air
9 Add the PEEK fitting to the needle tip and fix both at the flow cell inlet.
Do not inject the calibration sample without the sample filter.
NO TE
10 Slowly inject about 0.2 ml and wait for about 10 seconds to inject another
1100 Series FD Reference Manual
Syringe with Sample Filter
out of the syringe and to flush the needle.
0.1 ml. This will assure that the cell is filled properly.
Troubleshooting and Test Functions
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