Flow Cell Flushing - Agilent Technologies 1100 Series Reference Manual

Flow Cell Flushing

When required
Tools required
Parts required
Aqueous solvents in the flow cell can built up algae. Algae do fluoresce. Therefore do not
NO TE
leave aqueous solvents in the flow cell for longer periods. Add a small percentage of
organic solvents (e.g. Acetonitrile or Methanol ~5%).
In case the cell is contaminated, follow the procedure below.
Flushing Procedure
1 Flush with bidistilled water.
2 Flush with nitric acid (65%) using a glass syringe.
3 Leave this solution in the cell for about one hour.
4 Flush with bidistilled water.
This concentration of nitric acid is dangerous and proper attention to safety should be
CAU TI O N
given. Also the nitric acid flushing procedure is not an infallible remedy for a dirty cell.
It is to be used as a last attempt to salvage the cell before cell replacement. Note that
the cell is a consumable item.
Do not exceed the pressure limit of 20 bar (0.2 MPa).
NO TE
1100 Series FD Reference Manual
If flow cell is contaminated
Glass syringe, adapter
Bidistilled water, nitric acid (65%), tubings to waste
Repairing the Fluorescence Detector
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