Agilent Technologies 1100 Series Reference Manual page 56

2
First Steps with the Fluorescence Detector
Evaluating The System Background
The example below uses water.
1 Pump solvent through your system.
2 Set the fluorescence scan range under FLD special setpoints according to
The scan time will increase when the range is enlarged. With the default values, the scan
NO TE
takes about 2 minutes.
3 Set PMT gain to 16.
The wavelength
range and step
number defines the
duration. Using the
maximum range, the
scan would take
approximately 10
minutes
Figure 24
4 Define a data file name and take a fluorescence scan. After the scan is
58
your needs.
FLD special settings
completed, the isoabsorbance scan results appear, see Figure 25 on page 59.
1100 Series FD Reference Manual