Agilent Technologies 1100 Series Reference Manual page 42

2
First Steps with the Fluorescence Detector
Table 7
Column
Mobile phase
Gradient
Flow rate
Column temperature
Injection volume
FLD settings
This shows the
isofluorescence plot
of emission spectra
for 15 PNAs
(5 µg/ml) with a
fixed excitation
wavelengths
(260 nm).
Figure 13
44
Conditions for Figure 13 and Figure 14 on page 45
Optimization of the time-program for the emission wavelength
Vydac, 2.1 x 200 mm, PNA, 5
A = water; B = acetonitrile (50:50)
3 minutes, 60%
14 minutes, 90%
22 minutes, 100%
0.4 ml/min
°
18 C
µ
5 l
PMT 12,
response time 4 s,
step size 5 nm
1100 Series FD Reference Manual
µ
m