Analytical Information From Primary Data; Fluorescence Detection - Agilent Technologies 1100 Series Reference Manual

Analytical Information From Primary Data

We now know how the primary data from your sample is acquired in the
optical unit. But how can the data be used as information in analytical
chemistry? Depending on the chemistry of your application, the luminescence
measured by the fluorescence detector will have different characteristics. You
must decide, using your knowledge of the sample, what mode of detection you
will use.

Fluorescence Detection

When the lamp flashes, the fluorescing compounds in your sample will
luminesce almost simultaneously, see
short-lived, therefore the fluorescence detector need only measure over a
short period of time after the lamp has flashed.
Figure 83
1100 Series FD Reference Manual
Intensity
100%
Ignite
0
Measurement of Fluorescence
Introduction to the Fluorescence Detector
Figure
1 2
Time [µsec]
83. The luminescence is
Track&Hold
3
7
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