Flow Cell Flushing - Agilent Technologies Infinity 1260 User Manual

Flow Cell Flushing

When If flow cell is contaminated
Tools required Glass syringe, adapter
Parts required #
1
Dangerous solvents
WA R N I N G
The strong solvents used in this procedure are toxic and flammable and proper
precautions are necessary.
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Aqueous solvents in the flow cell can build up algae. Therefore do not leave aqueous
N O T E
solvents in the flow cell for long periods. Add a small percentage of organic solvents (e.g.
Acetonitrile or Methanol ~ 5%).
The strong solvent should dissolve any potential contaminants in the flow cell. For example
N O T E
water for aqueous mobile phase buffers, chloroform or tetrahydrofuran for not water
soluble contaminants.
In case the cell is contaminated, follow the procedure below.
1 Flush with the strong solvent.
2 Leave this solution in the cell for about one hour.
3 Flush with mobile phase.
Do not exceed the flow cell pressure limit of 5 bar (0.5 MPa).
N O T E
Agilent 1260 Infinity RID User Manual
Description
Strong solvent, tubings to waste
Wear protective gloves and goggles.
Don't expose yourself to the vapors.
9
Maintenance

Flow Cell Flushing

135