Agilent Technologies 1100 Series Reference Manual page 48

2
First Steps with the Fluorescence Detector
The upper trace was
received with
traditional wavelength
switching.
Figure 17
Previously, only diode array detectors and mass spectrometric detectors could
deliver spectral information on-line to confirm peak identity as assigned by
retention time.
Now, fluorescence detectors provide an additional tool for automated peak
confirmation and purity control. No additional run is necessary after the
quantitative analysis.
During method development, fluorescence excitation and emission spectra are
collected from reference standards and entered into a library-at the choice of
the method developer. All spectral data from unknown samples can then be
compared automatically with library data. Table 3 illustrates this principle
using a PNA analysis. The match factor given in the report for each peak
indicates the degree of similarity between the reference spectrum and the
spectra from a peak. A match factor of 1,000 means identical spectra.
50
Simultaneous multi wavelength detection for PNA-analysis
1100 Series FD Reference Manual