Wavelength Verification And Calibration - Agilent Technologies 1100 Series Reference Manual

Wavelength Verification and Calibration

The wavelength calibration is based on a Glycogen solution, which acts as a
strong elastic light scatterer (refer to ASTM Test Method E388-72-1993
"Spectral Bandwidth and Wavelength Accuracy of Fluorescence
Spectrometers"). The Glycogen solution is introduced into the flow cell and
then the built-in wavelength calibration functionality is used.
The algorithm is based on evaluating different grating orders and calculating
the wavelength scales of both, excitation and emission monochromator, by
applying the fundamental grating equation.
A complete wavelength calibration is not always required. In most cases a quick
NO TE
wavelength accuracy verification is sufficient enough, see the table below.
Table 18
interest
GLP compliance
cell change
lamp change
monochromator change
main board change
optical unit change
( X ) only required, if deviation is too large.
Prior to a wavelength calibration, a wavelength accuracy verification should be performed,
NO TE
see
wavelength calibration should be done as described in
Procedure"
1100 Series FD Reference Manual
Reasons for doing a Verification or Calibration
"Check of Raman Band of Water"
on page 110.
Troubleshooting and Test Functions
Verification
X
X
X
X
on page 104. If the deviation is more than
WL calibration
( X )
( X )
X
X
X
"The Wavelength Calibration
4
±
3 nm, the
101