Agilent Technologies 1100 Series Reference Manual page 100

Fluorescence detector
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4
Troubleshooting and Test Functions
The duration of the wavelength calibration is about 15 minutes plus setup time for the
NO TE
calibration sample and system. Depending on the maximum intensity found during this
scan, the PMT gain will be changed automatically and requires an additional 1 minute per
scan.
Table 19
calibration.
The excitation grating and the emission grating are calibrated using Rayleigh
stray light from the flow cell or cuvette measured with the photomultiplier
tube.
Figure 33
102
on page 103 shows the steps performed during the wavelength
Wavelength Calibration - User Interface
1100 Series FD Reference Manual

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