Agilent Technologies 1100 Series Reference Manual page 101

Table 19 Wavelength Calibration Steps
Step Description
1 Preparation
2 Excitation rotation scan, full circle
3 Excitation rotation scan, high resolution
4 Excitation position scan, low resolution
5 Excitation position scan, high resolution
6.n Emission rotation scan, full circle
(# of scans depends on the required PMT gain, 1 minute per scan)
6.n Em rotation scan, full circle" (instrument profile)
6.n Em rotation scan, full circle" (instrument profile)
6.n Em rotation scan, full circle" (instrument profile)
6.n Em rotation scan, full circle" (instrument profile)
7 Emission rotation scan, high resolution, part I
8 Emission rotation scan, high resolution, part II
9 Emission position scan, low resolution
10 Emission position scan, high resolution
Notes:
1 Variable times means that they could be a little bit longer.
2 Steps "6.n Em rotation scan, full circle" (instrument profile)" have been
When the lamp is of, the calibration process will stop within the first two steps with "
NO TE
Wavelength Calibration Failed" , see
1100 Series FD Reference Manual
added with firmware revisions A.03.64 .
Troubleshooting and Test Functions
"Wavelength Calibration Failed"
4
Duration
max 30 s
60 s
44 s
55 s variable
260 s variable
61 s variable
9 s
9 s
9 s
9 s
44 s
44 s
50 s variable
250 s variable
on page 97.
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