How The Detector Operates - Agilent Technologies 1100 Series Reference Manual

How the Detector Operates

Luminescence Detection
Luminescence, the emission of light, occurs when molecules change from an
excited state to their ground state. Molecules can be excited by different forms
of energy, each with its own excitation process. For example, when the
excitation energy is light, the process is called photoluminescence.
In basic cases, the emission of light is the reverse of absorption, see
With sodium vapor, for example, the absorption and emission spectra are a
single line at the same wavelength. The absorption and emission spectra of
organic molecules in solution produce bands instead of lines.
Figure 73
1100 Series FD Reference Manual
Absorption of Light Versus Emission of Light
Introduction to the Fluorescence Detector
7
Figure 73.
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