7
Introduction to the Fluorescence Detector
Reference System
A reference diode, located behind the flow cell, measures the excitation (EX)
light transmitted by the flow cell and corrects flash lamp fluctuations and
long-term intensity drift. Because of a non-linear output of the diode
(depending on the EX-wavelength), the measured data are normalized.
A diffuser is located in front of the reference diode (see
page 244). This diffuser is made of quartz, reduces light and allows integral
measurement of the light.
250
Figure 77
on
1100 Series FD Reference Manual