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Normalization calibration
When to perform
the calibration
About the
calibration data
Preparing the
calibration array
card
Perform the
normalization
calibration
QuantStudio
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
™
12. Repeat the calibration to calibrate the instrument for the remaining dyes that are
present in the experiments that you will be running.
13. (Optional) Click Print Report in the upper right corner of the screen to print a
summary of the calibration results for your records.
(TaqMan
During the normalization calibration, the instrument collects and stores the data from
the normalization standards.
IMPORTANT! Normalization calibration is only required for the TaqMan
Card block. Normalization calibration is not required for the 96-well plate blocks or
the 384-well plate block.
For the TaqMan
®
Array Card block, perform a normalization calibration once every
6 months.
During the normalization calibration, the software captures a series of images of each
normalization array card using each instrument filter. The normalization calibration
yields a "Pass" or "Fail" result for each normalization consumable used.
IMPORTANT! Wear powder-free gloves and safety glasses when you prepare the
array cards.
For detailed instructions to prepare the calibration array card, see "Preparing array
cards for instrument calibration" on page 37.
Normalization calibration is only required for the TaqMan
1. In the QuantStudio
Console.
2. In the Instrument Console, select the icon for your instrument, then click
Manage Instrument.
Note: If the Manage Instrument button is inactive, add your system to the My
Instruments group.
3. In the Instrument Manager, start the calibration:
a. Click
Maintenance, then click Normalization.
b. In the Normalization Calibration screen, click Start Calibration.
4. In the Normalization Calibration screen, select the reporter and passive dye
combination that you want to calibrate, then perform the calibration as
instructed.
Normalization calibration (TaqMan
Array Card only)
®
™
Real-Time PCR Software Home tab, click Instrument
Chapter 2 Calibrate the instrument
Array Card only)
®
®
Array Card block.
2
®
Array
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