Ms Manual Control - Teledyne ACCQPrep HP125 Installation And Operation Manual

5.2.12 MS Manual Control

detected. The red spectrum is reset after injecting a
sample. To inject a sample, move the PurIon injection
valve to the "inject Sample position"
• Inject the sample (>20 uL) and move the valve to the
"Scan Mass" position.
Note
Only use 22 gauge square tip needles (PN 29-9001-911) to
avoid scratching the internal surfaces of the injection valve! Fil-
ter the sample with a 0.45 µ filter to avoid clogging the PurIon
tubing and probe capillary. Sample concentration should be
less than 20 µg/mL.
• Format Graph—Set the displayed range of the mass
spectrum. The PurIon system still collects the entire
mass range even when the range is set within than the
minimum and maximum values allowed for the
spectrometer. Values can range from 10 to 1200 Da
(PURION or PURION S) or 2000 Da (PURION L).
Changing the mass range defined by these controls may
cause the Y-axis (ion count) scaling to change based on
the tallest peak within the range. The display only
labels the m/z for the 10 most prevalent ions currently
displayed. Formatting the graph to a narrower range,
causes the system to relabel the ions to identify ions
that may not have been intense enough to be labeled on
the broader range graph.
• Spectrum Name- the file name for the mass spectrum is
only needed if you want to save the mass spectrum to a
file for later viewing.
• Save Data- saves the mass spectral data to the internal
hard drive.
• Save as PDF - save the displayed spectrum on either a
USB drive or a remote computer's hard drive. The file
name will be the same as the spectrum name except it
has a PDF extension. PeakTrak displays a file download
window so you can select a location and change the file
name.
• Fluid Interface Pressure - The carrier solvent pressure
is displayed in a ribbon gauge. If the pressure
approaches the level of the red line, the system is
clogged and should be cleaned to allow proper operation.
The MS->M C
ANUAL ONTROL
interface carrier solvent pump to be run for priming and to purge
the carrier solvent. This window can also be used for trouble
shooting.
• Start Carrier Pump- Turns the carrier pump on for
priming or to replace one carrier solvent with another
one. A purge is complete in ~5 minutes. This changes to
read S C
TOP ARRIER
ACCQPrep HP125
Section 5 PeakTrak
menu command allows the fluid
P when the pump is running.
UMP
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