Alternative Ways To Create Method Files; Defining A Gradient - Teledyne ACCQPrep HP125 Installation And Operation Manual

ACCQPrep HP125
Section 5 PeakTrak
5.3.2 Alternative Ways to
Create Method Files

5.3.3 Defining a Gradient

5-30
• Detection Ions: Sets ions for detection or to be
monitored. Up to 4 single ions may be chosen, or a range
of ions and up to 3 single ions may be chosen. On PurIon
S and PurIon L systems, detected ions may be a mixture
of positive and negative ions.
• Ion Settings: Sets ionization parameters to enhance
detection of molecular ion peaks.
Purity Measure – If using two absorbance wavelengths,
check this option to display a ratio of the selected wave-
lengths. The ratio trace is often a useful indicator of purity.
Use the SHOW SPECTRAL PURITY control to measure
purity by using a comparison of UV-spectra measured at
differing times as a purity measurement. The S
P D button allows fractionation based on
URITY ETECTION
spectral purity.
Saving Changes to the Method File – After you have
edited the method file, you can save the changes for future
use. Click the S A
AVE
descriptive name, then click the S
file will be stored by the ACCQPrep system and will be
available for future runs.
Other than editing a method file on the ACCQPrep system, there
are more ways to create method files.
Importing Method Files from another System – If a
method file is located on another ACCQPrep system, you
can use the FILE>EXPORT METHOD menu command to
save the method file on an external storage device. From
the touch screen panel, this storage device is a USB Flash
Drive connected to the USB port below the display. If con-
nected to the system via an Internet browser, the storage
device may be any device that can be accessed by the local
computer.
To move the method file onto the system, insert the USB
flash drive into the USB port or connect to the system from
the remote computer, then use the FILE>IMPORT
METHOD menu command to locate and import the method
file.
Extracting a Method File from a Previous Run – After
the run, you can extract the run parameters as a method
file so it can be used on future runs. To do so, open the Run
file and click the E
XTRACT
new method with identical run parameters. You can then
save the method using the F
commands.
The simplest way to change the gradient is to click and drag the
inflection points that define the shape of the gradient. Add a
point by clicking the I
NSERT
button, give the method file a
S
button. The method
AVE
button. The system will load a
>S M
ILE AVE ETHOD
button, then click the gradient curve
PECTRAL
A menu
S