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CombiFlash NextGen Systems
Section 3 Operation
3.2 Loading a RediSep
Column
3.3 Starting a Separation
3-2
The CombiFlash NextGen system has redundant safety
devices to limit pressure to less than 300 psi (2068 kPa);
150 psi (1034 kPa) for systems without an automated
injection valve. RediSep columns smaller than 100 g are CE
certified using standard IEC61010-1 for use on the NextGen
system. RediSep columns larger than 100 g meet Pressure
Vessel Directive 97/23/EC.
Note
For best results, always use RediSep columns. The system will
not automatically detect other columns. The system also limits
the maximum operating pressure to 100 psi when the column
is not detected.
To load a RediSep column:
1. Select the proper RediSep column. The RediSep column
selection guide that can assist you in selecting a stationary
phase media and column size. In addition, the approxi-
mate sample loading capacity is printed on the RediSep
column label.
2. Raise the column mount and insert the column into the top
column mount. Note that the column fittings are keyed to
ensure the correct flow direction.
3. Slowly lower the injection valve while aligning the bottom
column fitting. The spring-loaded column mount will hold
the column in place.
4. To seal the column fittings, give the column a slight twist
1
(
/
turn).
4
Note
After loading a RediSep column, systems configured with
RFID will use RFID technology to automatically detect the
media type and column size (if available on the column).
PeakTrak displays the detected column size on the M
M
E
windows. If the system does not detect the
ETHOD
DITOR
column, manually select the column media and size.
After completing the system installation steps, preparing the
sample (See the CombiFlash NextGen Quick Start Guide for
sample loading procedures), and inserting the column, you are
ready to perform a run. The system will assume a default method
based on the column size placed in the system. Default methods
are optimized for the use of RediSep columns with run param-
eters typically used by chemists.
EAR99 Technology Technology Subject to Restrictions Contained on the Cover Page
WARNING
and
AIN
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