Starting A Separation; Separation With An Autoinjector - Teledyne ACCQPrep HP125 Installation And Operation Manual

ACCQPrep HP125
Section 4 Operation

4.3 Starting a Separation

For an ELSD
For a PurIon
If No AutoInjector or
AutoSampler are
Connected
4.3.1 Separation with an
AutoInjector
4-2
To separate a single sample, press P
The M R R
INIMUM UN
allows you to select an injection technique and select where the
fractions will be collected (the starting rack and tube position). In
addition, the system projects the amount of solvent required and
the separation duration. To proceed from this screen, the S
E button is selected.
QUILIBRATION
If an ELSD is installed on the system, this screen has the option
of using the ELSD or disabling the ELSD. ELSD method param-
eters are typically based on the solvents used and therefore are
somewhat universal when doing preparative separations of non-
volatile samples. Semi-volatiles may require optimization of the
ELSD parameters. Peak delay compensation is based on using
water and methanol or water and acetonitrile and is selected
based on the names assigned to the solvents.
Note
The M E
ETHOD DITOR
parameters.
If a PurIon mass spectrometer is connected, the R
screen allows some customization of the PurIon operating
MENTS
conditions. This allows the use of default or commonly used
methods while allowing customization of the separation for both
masses used for fractionation or mass loading of sample sent to
the PurIon for detection. (Low mass load is the default and sends
the minimum amount of sample to the PurIon for detection. Due
to the high concentrations of sample in a preparative separation
this is adequate for most samples with good ionization.) Further
information can be found in Section 5.2.11.
If you do not have an AutoInjector or AutoSampler, the
ACCQPrep will give you the option of injecting the sample imme-
diately or after column equilibration.
• L N - If your sample is ready you can inject it into
OAD OW
the syringe port on the side. Press S
, and walk away.
BRATION
• L A E
OAD FTER QUILIBRATION
give your column time to equilibrate while you finish
preparing your sample. Once the equilibration is
complete, the system will pause and prompt you to inject
your sample.
The next section will cover how to start a separation when the
additional modules are set up.
To use the AutoInjector Module:
1. Place the sample probe into your sample container.
2. Press P to start a separation as you would for an indi-
LAY
vidual separation.
to start a separation.
LAY
screen is displayed. This
EQUIREMENTS
should be used to change the ELSD
TART
- Select this if you want to
TART
R -
UN EQUIRE
E -
QUILI