Method Comparison With Human Whole Blood Samples - Siemens Rapidlab 1200 Operator's Manual

E-68
Table E-65 Rapidlab 1265 CO-ox Module Recovery and Precision with Total
Hemoglobin of ~20 g/dL and Oxyhemoglobin > 75%
Analyte
FHHb
* tHb mode performance is equivalent to sample device used
† WRSD: within-run standard deviation

Method Comparison with Human Whole Blood Samples

For testing syringe and capillary modes, blood was collected in heparinized vacuum tubes.
It was tonometered at 37°C and/or adjusted chemically. Several levels of tHb were
prepared by separating the red cells from the plasma and recombining appropriately.
Specimens were assayed on Rapidlab 1265 and Rapidlab 865 systems.
Method comparison was quantified by obtaining estimates of proportional (slope), and
constant (intercept) bias, root mean square error (RMSE), and method correlation (R
square for r
analysis of the Rapidlab 1265 (dependent variable) versus the Rapidlab 865 (independent
variable). Results are summarized in the table below.
Table E-66
Mode
Capillary
Syringe
Micro-
capillary
Micro-
syringe
pH Only
Capillary
pH Only
Syringe
pH, Glu,
Lac
Capillary
pH, Glu,
Lac
Syringe
Table E-67
Mode
Capillary
Syringe
02087462 Rev.V
Rapidlab 1200 Operator's Guide: System Specifications
*
Mode n
Syringe 62
2
coefficient of determination) by performing an ordinary least squares
Rapidlab 1265 Human Whole Blood Method Comparison—pH
N Slope Intercept
117 0.996 0.036
259 1.005 -0.030
71 0.986 0.104
145 0.991 0.064
122 1.003 -0.020
243 1.006 -0.051
138 0.986 0.106
274 0.991 0.070
Rapidlab 1265 Human Whole Blood Method Comparison—pCO
N Slope Intercept
198 1.014 0.211
410 0.958 2.020
Mean
Reference Mean Bias
4.53 -0.52
2
RMSE r
0.006 0.999
0.009 0.998
0.006 0.999
0.006 0.999
0.008 0.998
0.008 0.998
0.009 0.998
0.008 0.998
2
RMSE r
3.179 0.998
4.038 0.996
†
WRSD
0.22
Min Max
7.052 7.691
7.010 7.696
7.020 7.643
7.022 7.646
7.017 7.643
7.012 7.645
7.044 7.652
7.054 7.661
2
Min Max
7.0 238.2
6.1 233.9