Method Comparison With Human Whole Blood Samples - Siemens Rapidlab 1200 Operator's Manual

E-28

Method Comparison with Human Whole Blood Samples

For testing syringe and capillary modes, blood was collected in heparinized vacuum tubes.
It was tonometered at 37°C or adjusted chemically. Several levels of tHb were prepared by
separating the red cells from the plasma and recombining appropriately. Specimens were
assayed on Rapidlab 1245 and Rapidlab 865 systems.
Method comparison was quantified by obtaining estimates of proportional (slope), and
constant (intercept) bias, root mean square error (RMSE), and method correlation (R
square for r
analysis of the Rapidlab 1245 (dependent variable) versus the Rapidlab 865 (independent
variable). Results are summarized in the table below.
Table E-24
Mode
Capillary
Syringe
Micro-
capillary
Micro-
syringe
pH Only
Capillary
pH Only
Syringe
Table E-25
pCO
2
Mode
Capillary
Syringe
Micro-
capillary
Micro-
syringe
Table E-26
Mode
Capillary
Syringe
Micro-
capillary
02087462 Rev.V
Rapidlab 1200 Operator's Guide: System Specifications
2
coefficient of determination) by performing an ordinary least squares
Rapidlab 1245 System Human Whole Blood Method Comparison—pH
N Slope Intercept
135 1.002 -0.013
288 1.008 -0.059
144 0.988 0.089
144 1.003 -0.029
144 1.018 -0.123
288 1.014 -0.096
Rapidlab 1245 System Human Whole Blood Method Comparison—
N Slope Intercept
215 0.961 1.609
429 0.962 1.618
108 1.004 1.044
216 0.991 -1.479
Rapidlab 1245 System Human Whole Blood Method Comparison—pO
N Slope Intercept
215 0.974 4.195
429 0.984 2.932
84 0.922 8.530
2
RMSE r
0.006 0.999
0.007 0.999
0.015 0.994
0.007 0.999
0.009 0.998
0.007 0.999
2
RMSE r Min
5.348 0.993 6.7
5.087 0.994 6.7
3.225 0.998 6.9
4.363 0.996 6.7
2
RMSE r Min
12.240 0.996 27.6
11.912 0.996 25.4
5.706 0.993 26.7
Min Max
7.0 7.7
7.0 7.7
7.0 7.7
7.0 7.6
7.0 7.7
7.0 7.7
Max
231.4
241.8
237.3
242.0
Max
677.3
687.4
254.3
2