Method Comparison With Human Whole Blood Samples - Siemens Rapidlab 1200 Operator's Manual

E-46
Table E-42
Level Mode
4
5
* WRSD = within-run standard deviation

Method Comparison with Human Whole Blood Samples

For testing syringe and capillary modes, blood was collected in heparinized vacuum tubes.
It was tonometered at 37°C and/or adjusted chemically. Several levels of tHb were
prepared by separating the red cells from the plasma and recombining appropriately.
Specimens were assayed on Rapidlab 1260 and Rapidlab 865 systems.
Method comparison was quantified by obtaining estimates of proportional (slope), and
constant (intercept) bias, root mean square error (RMSE), and method correlation (R
square for r
analysis of the Rapidlab 1260 (dependent variable) versus the Rapidlab 865 (independent
variable). Results are summarized in the table below.
Table E-43
Mode
Capillary
Syringe
Micro-
capillary
Micro-
syringe
pH Only
Capillary
pH Only
Syringe
pH, Glu,
Lac
Capillary
pH, Glu,
Lac
Syringe
02087462 Rev.V
Rapidlab 1260 Recovery and Precision Testing—Lactate
N WRSD
pH, Glu, 46 0.15
Lac
Syringe
pH, Glu, 47 0.36
Lac
Syringe
2
coefficient of determination) by performing an ordinary least squares
Rapidlab 1260 Human Whole Blood Method Comparison—pH
N Slope
145 1.001 -0.003
284 1.015 -0.108
72 0.989 0.080
143 0.950 0.361
143 1.001 -0.003
273 1.004 -0.035
144 1.001 -0.000
253 1.006 -0.045
Rapidlab 1200 Operator's Guide: System Specifications
*
Observed Expected
11.3 12.6
15.6 18.0
Intercept RMSE
0.009
0.008
0.006
0.059
0.007
0.009
0.007
0.006
Recovery
89.8
86.6
2
r Min Max
0.998 7.042 7.684
0.998 6.991 7.676
0.999 7.030 7.651
0.911 7.030 7.651
0.999 7.015 7.639
0.998 7.004 7.642
0.999 7.046 7.657
0.999 7.045 7.660
WRCV
1.3
2.3