Wavelength Verification And Calibration - Agilent Technologies 1260 Infinity User Manual

8
Test Functions

Wavelength Verification and Calibration

Wavelength Verification and Calibration

The wavelength calibration is based on a Glycogen solution, which acts as a
strong elastic light scatterer (refer to ASTM Test Method E388-72-1993
"Spectral Bandwidth and Wavelength Accuracy of Fluorescence
Spectrometers"). The Glycogen solution is introduced into the flow cell and
then the built-in wavelength calibration functionality is used.
The algorithm is based on evaluating different grating orders and calculating
the wavelength scales of both, excitation and emission monochromator, by
applying the fundamental grating equation.
A complete wavelength calibration is not always required. In most cases a quick
N O T E
wavelength accuracy verification is sufficient enough, see
Table 33
interest
GLP compliance
cell change
lamp change
monochromator change
main board change
optical unit change
( X ) only required, if deviation is too large.
Prior to a wavelength calibration, a wavelength accuracy verification should be performed,
N O T E
see
wavelength calibration should be done as described in
Procedure"
164
Reasons for doing a Verification or Calibration
"Wavelength Accuracy Test"
on page 173.
Verification
X
X
X
X
on page 167. If the deviation is more than ±3 nm, the
Table 33 on page 164.
WL calibration
( X )
( X )
X
X
X
"Wavelength Calibration
Agilent 1260 FLD User Manual