Reference System - Agilent Technologies 1200 Series User Manual

Introduction to the Fluorescence Detector 1

Reference System

A reference diode, located behind the flow cell, measures the excitation (EX)
light transmitted by the flow cell and corrects flash lamp fluctuations and
long-term intensity drift. Because of a non-linear output of the diode
(depending on the EX-wavelength), the measured data are normalized.
A diffuser is located in front of the reference diode (see Figure 6 on page 15).
This diffuser is made of quartz, reduces light and allows integral measurement
of the light.
1200 Series FLD User Manual 21