Installing A Trap Column; Connecting The Components To The Column Switching Valve - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

UltiMate 3000 Series:
NCS-3500RS and NCP-3200RS

4.6.3 Installing a Trap Column

Certain applications, for example, preconcentration, require the installation of a trap
column. The trap column is connected directly to the column switching valve.
When installing the trap column, observe the direction of flow (indicated by an arrow on the
column). Connect the column to the valve in the direction of the nano flow. More information
about how to connect the column is shipped with the column. In addition, observe the general
information about how to connect capillaries (→ page 57).

4.6.4 Connecting the Components to the Column Switching Valve

1. Do not touch any metal or plastic parts inside the column chamber while the
temperature is higher than 50 °C. Before carrying out any work in the column
compartment, wait for the column chamber to cool down.
To shorten the cool-down time, consider setting a lower temperature and opening the
front panel door.
2. A plastic cap protects the valve ports during shipment. Remove the plastic cap if it is
still present.
3. Connect the capillaries to the column switching valve as required by your application.
When connecting the capillaries, observe the general information about how to
connect and route the capillaries (→ pages 57 and 60).
Page 82 Operating Instructions