Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual page 64

UltiMate 3000 Series:
NCS-3500RS and NCP-3200RS
7. Pump Module
a) Set up the seal wash system (→ page 67) and flush the system with the seal wash
solution (→ page 67).
b) This step depends on the flow meter that is installed:
♦ Classic flow meter
Specify the viscosity of the solvents used with the NC pump (→ page 69).
♦ ProFlow flow meter
Set the solvent types for the solvents used with the NC pump (→ page 69).
Keep in mind that the module can deliver exact flow rates and gradients only if the
viscosity (Classic flow meter) or solvent type (ProFlow flow meter) is set correctly.
c) Purge the pumps of the pump module (→ page 72).
d) Check and change the leak sensor setting if necessary (→ page 124).
e) Adjust the brightness and contrast of the front panel display if necessary
(→ page 125).
8. Column Compartment
a) Install the valves if required (→ page 78).
b) Install the separation columns (→ page 80).
c) Install a trap column if required for your application (→ page 82).
d) Establish the fluid connections between the columns and switching valves, as required
by your application. For information about how to connect the capillaries to the valve,
see section 4.6.4 (→ page 82).
e) Turn on temperature control if applicable and set the temperature setpoint
(→ page 127).
f) Activate column identification if applicable (→ page 129).
g) Check and change the sensitivity settings for the gas and humidity sensors if necessary
(→ page 130).
h) Adjust the brightness and contrast of the front panel display if necessary
(→ page 125).
9. Connect the module to the other modules of your RSLCnano system, as required by
your application. When connecting the capillaries, observe the general information on
page 57.
10. Before starting sample analysis, equilibrate the entire system (→ page 83).
Page 56 Operating Instructions