Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual page 131

Nano/cap system. nano/cap pump
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5.5.7.3 What happens ....
Correct functioning of the rear seal wash system
During the delivery period of the peristaltic pump, liquid reaches the detector of the rear seal
wash system. This means that the seal wash system performs correctly. The tubing is all right
and the peristaltic pump works correctly.
Malfunctioning of the rear seal wash system
If no drops reach the detector after maximum five minutes although the peristaltic pump is
pumping, this may indicate that the
Seal wash reservoir is empty.
Peristaltic tubing is blocked or crimped.
The seal wash tubing (silicone tubing) is pinched.
Detector of the system is dirty.
In all cases, the following message appears in the Chromeleon Audit Trail: "Rear seal wash
system has run out of wash solution". If the rear seal liquid is not discharged properly to the
waste, the following message may appear: "The rear seal leak sensor detects drops
constantly".
Take the following remedial action:
Check the seal wash reservoir level.
Replace the peristaltic tubing.
Verify that the liquid can pass the tubing properly. Replace the tubing if required.
Clean the detector electrodes (→ page 181).
Tip: If the seal wash reservoir is empty, the RearSealWashDry property in
Chromeleon (Commands dialog box) reports Dry. To start a batch or set the
pump flow nevertheless, set OverrideRearSealDry to Enabled. However,
note that the property will be automatically reset to Disabled after each wash
cycle.
Possible leakage of the main piston seal
If the message "Piston seal leakage has exceeded the recommended limit" appears, this
indicates possible leakage of the main piston seals.
Take the following remedial action:
Inspect the piston seals for leakage (→ page 186).
Replace the piston seals (→ page 194).
Operating Instructions
UltiMate 3000 Series:
NCS-3500RS and NCP-3200RS
Page 123

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Ncp-3200rsNcs-3500rs

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