Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual page 85

To purge the pump from Chromeleon
a) In Chromeleon, open the Commands dialog box for the loading pump.
The properties and commands for purging the loading pump appear under
PumpModule > LoadingPump.
b) Set the channel to be purged to 100% (for example, %A).
In Chromeleon, you cannot set %A directly. Thus, if %A be 100%, you have to set all
other components of the eluent to 0%.
c) Start the purge cycle. To do so, set Purge to On.
The loading pump is purged with settings specified for PurgeFlow and PurgeTime.
The standard settings are as follows: PurgeFlow = 2 mL/min, PurgeTime =
5 minutes.
When the PurgeTime has elapsed, Purge is automatically reset to Off. You can stop
purging also manually by setting Purge to Off.
d) Repeat the purge cycle for all channels of the loading pump (even if they are not used
for the application) until all air bubbles are gone.
To purge the pump from the pump display
a) On the pump display, select the Control menu for the loading pump (→ page 101).
b) Set the channel to be purged to 100% (for example, %A).
Start the purge cycle. To do so, set Purge to On.
The pump is purged with the Purge Flow and Purge Time settings from the
Preferences menu (→ page 102). The standard settings are as follows: Purge Flow =
2 mL/min, Purge Time = 5 minutes.
c) Draw the liquid with the syringe.
d) When the Purge Time has elapsed, Purge is automatically reset to Off. You can stop
purging also manually by setting Purge to Off.
e) Repeat the purge cycle for all channels of the loading pump (even if they are not used
for the application) until all air bubbles are gone.
6. Close the purge valve.
Turn the purge screw only with your hand (use no tools). If the valve screw leaks, tighten
a little more. Overtightening the purge valve can damage the cap seal.
Operating Instructions
UltiMate 3000 Series:
NCS-3500RS and NCP-3200RS
Page 77